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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in many tumors, which ends up in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants had been claimed but thorough nucleotide sequence info is not really but available.

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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide typically consists of the epitope regarded via the antibody.

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This guidebook outlines further achievable optimization of mobile and immunoassay parameters to be sure the best possible results are...

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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...

Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody by itself, you can see which staining is certain.

Concentrate on Information Cyclin E belongs to your really conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E varieties a posh with and capabilities as a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary and is also degraded as cells development by means of S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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